Particle Technology
Magnetic Particles
· Sera-Mag® Magnetic Particles
Nonmagnetic Particles
· Power-Bind™ Streptavidin
Microparticles (SA-MP)
Polystyrene Particles
· OptiBind® Polystyrene
Microparticles (PS)
· OptiLink® Carboxylate-Modified
Polystyrene (CM)
Dyed Particles
· Color-Rich™
Dyed Polystyrene
· Fluoro-Max™ Fluorescent
Carboxylate-Modified
Sera-Mag® Magnetic Particles
Sera-Mag magnetic particles are based on patent (5,648,124), using 1µM magnetic carboxylate-modified base particles made by a core-shell process. The surface has a unique cauliflower-like structure with a high carboxylic acid content. They combine a fast magnetic response time with a large surface area and fast reaction kinetics. Sera-Mag are used in a wide variety of molecular biology, nucleic acid isolation, research protocols and clinical immunoassay reagent applications.

SpeedBeads™ Magnetic Particles [ Download PDF (279k) ]
The SpeedBeads are the newest entry in Seradyn’s Sera-Mag line of magnetic beads. The SpeedBeads respond twice as fast to a magnetic field as our original Sera-Mag particles, and are available in carboxylate-modified and streptavidin coated versions.

The entire line of Sera-Mag encapsulated magnetic particles exhibit high binding capacity, excellent stability, low nonspecific binding, exquisite sensitivity and very slow settling rates.

The new SpeedBeads will create improved immunoassay and molecular biology sample prep reagents. The increased speed of response to a magnetic field will allow shorter assay time in clinical diagnostic tests and faster particle movement through viscous solutions for molecular biology applications. They are especially useful in molecular biology sample preparation applications.

Magnetic Carboxylate-Modified (MG-CM) [ Download PDF (250k) ]
These microparticles are colloidally stable in the absence of a magnetic field. When a magnetic force is applied, the particles are separated rapidly and completely from suspensions. Covalent coupling (of proteins, nucleic acids, etc.) to carboxyl groups on the surface is easily accomplished using our standard coupling technology. To learn more about this procedure, download our Recommended Adsorption and Covalent Coupling Procedures (136k PDF) protocol.

Magnetic Streptavidin (MG-SA) [ Download PDF (255k) ]
Containing covalently bound streptavidin, these particles come in four levels of biotin binding capacity ranging from 2000-4500 pmol/mg. Testing of these particles shows excellent shelf-life up to 5 years.

Magnetic Oligo(dT) Particles (MG-OL) [ Download PDF (209k) ]
These particles contain covalently bound oligo(dT) which are colloidally stable in the absence of a magnetic field. Binding capacity is measured by binding of (dA)30 and is expressed as pmol of (dA)30 bound per mg of beads.

MG-OL particles are mainly used for mRNA isolation. MG-OL particles can also be used as a universal base particle for coupling unique oligo sequences. Simply synthesize your oligo with a poly-A tail for easy attachment to the oligo(dT) particles.

Solid Phase Reversible Isolation (SPRI®)
Solid Phase Reversible Immobilization is a patented, high performance nucleic acid purification technology developed at MIT’s Whitehead Institute (Hawkins, et. Al., Nucleic Acids Res. 1995; 23:22). SPRI is based on the selective immobilization of nucleic acids onto Sera-Mag magnetic particles. SPRI helped scientists to process the enormous number of purifications required for the Human Genome Project. It has been adopted by several of the largest genome research facilities, resulting in more base pairs contributed to GenBank® than any other nucleic acid purification technology.

SPRI has been successfully automated on the following systems
  • Biomek® FX (Beckman Coulter)
  • DNATrak™ (PerkinElmer)
  • Sciclone™ ALH 3000 (Zymark)
  • Evolution™ (PerkinElmer)
  • MultiPROBE® II (PerkinElmer)
  • Genesis™ (Tecan)
  • Biorobot® (Qiagen)
  • Molecular BioCube™ (Protedyne)

Seradyn | Particle Technologies
Standard Packaging
Most microparticle products are sold in 15 mL, 100 mL and 1000 mL packages at 10% solids. Dyed, fluorescent, magnetic and other specialty particles are available in different concentrations and package sizes. The particles are routinely supplied with 0.05% sodium azide.
Standard
Packaging		 % Solids 1 mL 5 mL 15 mL 100 mL 1000 mL Preservative Suggestive
Storage
OptiBind Polystyrene
Particles (PS)		 10      • • • N/A  2-8 °C
OptiLink
Carboxylate-Modified (CM)		 10     • • • N/A 2-8 °C
Fluoro-Max Fluorescent Particles (CMEu) 1 • • • •   0.05% Az 2-8 °C
Color-Rich Dyed (PS/CM) 2.5     • •   0.05% Az 2-8 °C
Power-Bind Steptavidin (SA-MP) 1 • •   •   0.05% Az 2-8 °C
Sera-Mag Magnetic Carboxyl (MG-CM) 5      • • • 0.05% Az 2-8 °C
Sera-Mag Magnetic Streptavidin
(MG-SA)		 1 • •   •   0.05% Az 2-8 °C
Sera-Mag Magnetic Oligo(dT) (MG-OL) 1 • •   •   0.05% Az 2-8 °C
+ Enlarge Image  
“The superior performance of SPRI® is the result of the unique properties of the chemistry and the highly-engineered Sera-Mag microparticles manufactured by Seradyn.”
–Agencourt Bioscience Corporation
· Microparticle Application
  & Usage Chart
· Material Safety Data Sheets
· Product Technical Notes
· Application Notes / Protocols
· Product Manuals
Unique Properties Stability For Use In
  • Low, non-specific binding of proteins and other substances
  • Non-leaching, encapsulated magnetite
  • Covalently bound ligands do not leach
  • Surfactant-free—no washing required
  • High surface area per unit mass
  • High ligand binding capacity
  • Low settling rate
  • Tight size distribution resulting in simultaneous magnetic separation
  • Excellent lot-to-lot reproducibility
  • Less expensive than silica
    		•
  • pH 4 – 12
  • Guanidinium thiocyanate
  • DMF and DMSO to 100%
  • Sonication environments
  • PCR temperature cycling
  • Minimum 30 month shelf-life
    		•  Molecular Biology
  • Solid Phase Reversible Isolation (SPRI®)
  • Plasmid and Genomic DNA isolation
  • mRNA and PNA isolation
  • Cell isolation
  • Biotinylated PCR product isolation
  • Gene expression analysis
  • Northern Blot analysis
  • cDNA library construction
  • Sequence reaction clean up
  • Base particle for poly-A modified oligo sequences

    Clinical Diagnostics
  • Chemiluminescent/colorimetric assays
  • Heterogeneous/homogenous assays
    		•

    Ordering Guide
    Magnetic Carboxylate-Modified (MG-CM) [ Download PDF (250k) ]
    Catalog #DiameterDescriptionCapacity
    2415-21051 µMHigh acid contentHigh
    4415-21051 µMHigh acid contentHigh

    Magnetic Streptavidin (MG-SA) [ Download PDF (255k) ]
    Catalog #DiameterDescriptionCapacity
    3015-21031 µMMedium biotin binding capacity~4000 pmol/mg
    3015-21041 µMHigh biotin binding capacity~4500 pmol/mg
    3015-21051 µMVery high biotin binding capacity>4500 pmol/mg

    Magnetic Oligo(dT) Particles (MG-OL) [ Download PDF (209k) ]
    Catalog #DiameterDescriptionCapacity
    2815-21031 µMHigh (dA)30 binding capacity> 300 pmol/mg

    SpeedBeads Magnetic Carboxyl Particles [Download PDF (279kb)]
    Catalog #DiameterDescriptionCapacity
    6515-21051 µMCarboxylated - High acid contentHigh
    4515-21051 µMCarboxylated - High acid contentHigh

    SpeedBeads Magnetic Streptavidin Particles [Download PDF (279kb)]
    Catalog #DiameterDescriptionCapacity
    6615-21041 µMStreptavidin - High biotin binding capacity~4000 pmol/mg

    Sample Evaluation Packs
    Some of our magnetic particle products are sold in economical sample evaluation pack form. These evaluation packs allow you to buy small samples from among our inventory of different binding capacities, surface blocked, or magnetic response times at greatly reduced prices.

    Sera-Mag® Magnetic Streptavidin (MG-SA) biotin binding capacity evaluation pack
    Packaged in 1 mL bottles at 1% solids (10 mg/mL). These are available in four different biotin binding capacities (and 1 additional surface blocked high biotin binding version). Your evaluation pack options are 3 to 5 samples of low to high biotin-binding capacity particles and the surface blocked version.

    SpeedBead™ Magnetic Streptavidin high biotin binding (and surface blocked) evaluation pack
    Packaged in 1 mL bottles at 1% solids (10 mg/mL). The 2 sample evaluation pack contains 1 mL each of:
    Sera-Mag SpeedBeads™ high biotin binding capacity particles (surface unblocked)
    Sera-Mag SpeedBeads™ high biotin binding capacity particles (surface blocked).

    Sera-Mag® Magnetic Carboxyl or SpeedBead™ carboxyl
    The evaluation pack contains 15 mL each of our original Sera-Mag carboxyl and SpeedBeads™ carboxyl particles.
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